Acetylcholinesterase induction by beta-ecdysone in Drosophila cell lines and their hybrids

Drosophila S3 line cells maintain a high basal level of acetylcholinesterase (AChE) activity, which is lost and then reinduced following exposure to the steroid, beta-ecdysone. Electrophoretic studies indicate that the basal and reinduced AChE activities may be different isozymes. In contrast, MDR line cells have a low basal AChE activity which is induced to a modest level by hormone. In S3/MDR hybrids the high basal level phenotype is extinguished, and the hormone-induced AChE level is modest. MDER line cells, which continue to grow in the presence of beta-ecdysone and are therefore termed resistant, show a low basal AChE level that is not further elevated by hormone. This phenotype and response also occurred in beta-ecdysone-resistant S3/MDER hybrids.