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[Pyocin production by bacillus pyocyaneus and sensitivity of strains of different origin to them]
Abstract:
Using the method proposed by Gillies and Govan and their indicator strains, 342 P. aeruginosa strains isolated from the patients were studied in respect to their pyocinogenicity and typed according to the production of different types of pyocins. Besides, in 206 cultures the pyocin sensitivity of 16 standard P. aeruginosa strains (5 strains obtained from Govan and 11 strains provided by the authors) was determined. All the tested cultures fell into 23 pyocin types; of these, types I and X occured most frequently, 56 strains identified by means of indicators could not be typed due to the fact that the corresponding pyocin types were absent in Govan's scheme. The cultures isolated from the patients and the environmental objects during the outbreak of P. aeruginosa in a hospital were proved to belong to the same pyocin type (III). The double typing of the cultures, according to pyocin production and pyocin sensitivity, allowed to determine individual characteristics of 75% of the tested cultures.
Insights
This study characterized 342 Pseudomonas aeruginosa strains using pyocin typing. Most strains belonged to types I and X, with 75% individually identified by dual pyocin production and sensitivity typing.
Area of Science:
- Microbiology
- Bacteriology
- Clinical Microbiology
Background:
- Pseudomonas aeruginosa is an opportunistic pathogen.
- Pyocin typing is a method for differentiating bacterial strains.
- Understanding P. aeruginosa strain diversity is crucial for infection control.
Purpose of the Study:
- To pyocin type 342 P. aeruginosa strains isolated from patients.
- To determine the pyocin sensitivity of standard P. aeruginosa strains.
- To assess the utility of combined pyocin production and sensitivity typing for strain characterization.
Main Methods:
- Utilized the Gillies and Govan method for pyocin typing.
- Determined pyocinogenicity and pyocin sensitivity of P. aeruginosa strains.
- Employed 16 standard P. aeruginosa strains as indicators.
Main Results:
- Identified 23 distinct pyocin types among the 342 P. aeruginosa isolates.
- Pyocin types I and X were the most prevalent.
- Identified 56 strains that could not be typed using the existing scheme.
- Confirmed that P. aeruginosa strains from a hospital outbreak belonged to the same pyocin type (III).
- Dual typing (production and sensitivity) characterized 75% of the tested strains.
Conclusions:
- Pyocin typing is effective for differentiating P. aeruginosa strains.
- The existing pyocin typing scheme may require expansion to include new types.
- Combined pyocin production and sensitivity typing enhances strain characterization.
- This method aids in tracking P. aeruginosa during hospital outbreaks.