Isolation and quantitative immunoelectrophoresis of erythrocyte carbonic anhydrase B and C in subjects with thyroid disorders
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A simple procedure for separating carbonic anhydrase from hemoglobin in hemolysates is described. The procedure uses adsorption on diethylaminoethyl Sephadex at pH 8.7. The adsorbed material is hemoglobin and the carbonic anhydrases are isolated. The different isoenzymes A, B and C of carbonic anhydrase are separated from each other on another diethylaminoethyl Sephadex column by elution at pH 8.7. The pure isoenzymes are used for preparation of antibody in rabbits. It has been shown that concentration of HCA type B in erythrocytes increases in hypothyreosis and decreases in hyperthyreosis. Many drugs interact with the usual diagnostic tests used in the diagnosis of thyroid disorders. The purpose of this investigation was to examine, by an immunological method, the diagnostic value of HCA type B in distinguishing hyperthyroid subjects from euthyroid pregnant and oestrogen-treated subjects, who normally have thyroxine values in the hyperthyroid range.
A simple procedure for separating carbonic anhydrase from hemoglobin in hemolysates is described. The procedure uses adsorption on diethylaminoethyl Sephadex at pH 8.7. The adsorbed material is hemoglobin and the carbonic anhydrases are isolated. The different isoenzymes A, B and C of carbonic anhydrase are separated from each other on another diethylaminoethyl Sephadex column by elution at pH 8.7. The pure isoenzymes are used for preparation of antibody in rabbits. It has been shown that concentration of HCA type B in erythrocytes increases in hypothyreosis and decreases in hyperthyreosis. Many drugs interact with the usual diagnostic tests used in the diagnosis of thyroid disorders. The purpose of this investigation was to examine, by an immunological method, the diagnostic value of HCA type B in distinguishing hyperthyroid subjects from euthyroid pregnant and oestrogen-treated subjects, who normally have thyroxine values in the hyperthyroid range.