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[Sodium nucleinate increase of nonspecific macroorganism resistance to conditionally pathogenic and pathogenic
Abstract:
Sodium nucleinate significantly increased the non-specific resistance of mice to E. coli O26, Ps. vulgaris, Ser. marcesens, Ps. aeruginosa, Kl. ozaenae and their associations and total resistance also accompanied by a significant decrease in the number of the bacteria in the spleen and blood, the total number of the cells in the peritoneal exudate and the number of the cells adhering to the glass (macrophages). The preparation is a low molecular RNA consisting mainly of fraction 3S and a small amount of fraction 4S. It contains 1.5 per cent of protein and 2 per cent of DNA and does not contain any polysaccharides. Repeated purification of sodium nucleinate lowering the levels of the admixtures 5 times did not change its efficacy. The low molecular RNA of the rat liver (4S) had a pronounced stimulating activity. On infection of the host stimulated with sodium nucleinate, formation of the post-infection immunity was not decreased.
Insights
Sodium nucleinate, a low molecular ribonucleic acid (RNA), enhances mice's resistance to bacterial infections. This immune boost reduces bacterial load and inflammatory cell counts without hindering post-infection immunity.
Area of Science:
- Immunology
- Microbiology
- Biochemistry
Background:
- Non-specific resistance is crucial for combating bacterial infections.
- Understanding immune stimulants can lead to novel therapeutic strategies.
- Ribonucleic acid (RNA) derivatives show potential in modulating immune responses.
Purpose of the Study:
- To investigate the immunomodulatory effects of sodium nucleinate.
- To assess the impact of sodium nucleinate on non-specific resistance against various bacterial pathogens.
- To determine the composition and efficacy of sodium nucleinate.
Main Methods:
- Mice were challenged with Gram-negative bacteria (E. coli, Ps. vulgaris, Ser. marcesens, Ps. aeruginosa, Kl. ozaenae).
- Bacterial load in spleen and blood was quantified.
- Peritoneal exudate cells and macrophage adherence were analyzed.
- Sodium nucleinate composition (RNA, protein, DNA) was determined.
Main Results:
- Sodium nucleinate significantly enhanced resistance to multiple bacterial species.
- A significant decrease in bacterial counts in spleen and blood was observed.
- Total peritoneal exudate cells and macrophage adherence were reduced.
- The efficacy of sodium nucleinate was not affected by repeated purification.
Conclusions:
- Sodium nucleinate effectively boosts non-specific immunity against bacterial infections.
- Its mechanism involves reducing bacterial load and modulating inflammatory responses.
- Low molecular weight RNA (4S) exhibits significant stimulating activity.
- Sodium nucleinate does not impede the development of post-infection immunity.