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[Serotypes of Pseudomonas aeruginosa strains isolated from human sources in Diyarbakir (author's transl)]
Abstract:
951 Ps. aeruginosa strains were isolated from the patients at the hospital wards of Diyarbakir Medical Faculty. These strains were identified on the basis of methods employed by Gilardi. Anti-O typing sera were prepared with 17 Ps. aeruginosa strains obtained from Pasteur Institute of Paris. Ps. aeruginosa strains, isolated by slide agglutination and tube agglutination techniques were identified using Anti-O typing sera. According to these tests, Habs's strains 0:1, 0:3, 0:4, 0:7, 0:8, 0:9 0:10, 0:11, 0:12 and Sandvik's 0:13 and Veron's 0:2b, 0:2ab, 0:5d, 0:5cd strains were identified. Habs's strains 0:2, 0:5, 0:6 were not detectable. The most frequent type was 0:12 (26.39%) and the less frequent ones were 0:1 (2.31%) and 0:8 (2.31%).
Insights
This study identified Pseudomonas aeruginosa serotypes in hospital patients, finding O:12 to be the most common. These findings aid in understanding local strains for infection control.
Area of Science:
- Clinical Microbiology
- Bacteriology
Background:
- Pseudomonas aeruginosa (Ps. aeruginosa) is a significant nosocomial pathogen.
- Serotyping is crucial for epidemiological tracking and outbreak investigations of bacterial infections.
Purpose of the Study:
- To determine the serotype distribution of Ps. aeruginosa strains isolated from hospital patients in Diyarbakir.
- To provide data for infection control and surveillance strategies.
Main Methods:
- Isolation and identification of 951 Ps. aeruginosa strains from hospital patients.
- Serological identification using anti-O typing sera prepared with reference strains.
- Slide agglutination and tube agglutination techniques were employed.
Main Results:
- Seventeen different serotypes of Ps. aeruginosa were identified, including Habs's 0:1-0:4, 0:7-0:12, Sandvik's 0:13, and Veron's 0:2b, 0:2ab, 0:5d, 0:5cd.
- Serotype O:12 was the most prevalent, accounting for 26.39% of isolates.
- Serotypes O:1 and O:8 were the least frequent, each at 2.31%.
Conclusions:
- The study identified a diverse range of Ps. aeruginosa serotypes in a hospital setting.
- Serotype O:12 is the dominant type in this region, informing targeted prevention efforts.