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Related Experiment Videos

Radioimmunoassay method for baboon plasma gonadotropins.

J H Su, T Aso, T Motohashi

    Endocrinologia Japonica
    |August 1, 1980
    PubMed
    Summary

    Radioimmunoassay methods for baboon luteinizing hormone (bLH) and follicle stimulating hormone (bFSH) were developed. These assays accurately measure bLH and bFSH levels throughout baboon menstrual cycles.

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    Area of Science:

    • Reproductive Endocrinology
    • Primate Physiology
    • Hormone Assays

    Background:

    • Accurate measurement of reproductive hormones is crucial for understanding primate reproductive cycles.
    • Existing radioimmunoassay (RIA) methods may not be suitable for non-human primates like baboons.
    • Development of specific assays for baboon hormones is needed.

    Purpose of the Study:

    • To develop and validate double antibody radioimmunoassay (RIA) methods for quantifying baboon luteinizing hormone (bLH) and follicle stimulating hormone (bFSH).
    • To assess the influence of other hormones and releasing hormones on bLH and bFSH levels.
    • To characterize the patterns of bLH and bFSH during the baboon menstrual cycle.

    Main Methods:

    • Development of a homologous RIA for bLH using anti-ovine LH serum and radioiodinated ovine LH.

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  • Development of a heterologous RIA for bFSH using anti-ovine FSH serum and radioiodinated human FSH.
  • Validation using a crude rhesus pituitary extract as a reference standard and testing for cross-reactivity with other human hormones.

    • Main Results:

    • Established RIA methods for bLH and bFSH with no cross-reactivity from human prolactin (hPRL), human chorionic gonadotropin (hCG), human thyroid stimulating hormone (hTSH), or human growth hormone (hGH).
    • TRH administration did not affect LH and FSH levels, but LHRH combined with TRH increased LH and FSH.
    • Plasma LH and FSH concentrations in female baboons showed patterns similar to rhesus monkeys and humans during ovulatory cycles.

    Conclusions:

    • Developed specific and reliable RIA assays for bLH and bFSH in baboons.
    • Confirmed the distinct regulation of LH and FSH by LHRH and TRH in baboons.
    • Demonstrated conserved patterns of LH and FSH secretion during the menstrual cycle across primate species.