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Updated: Aug 9, 2026
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LERLIC-MS/MS for In-depth Characterization and Quantification of Glutamine and Asparagine Deamidation in Shotgun Proteomics
Published on: April 9, 2017
Acid-base balance and plasma glutamine concentration in man
Summary
Altered acid-base balance significantly impacts plasma glutamine levels. Both metabolic acidosis and alkalosis change glutamine concentrations, with PCO2 levels playing a key role in this regulation.
Area of Science:
- Physiology
- Biochemistry
- Acid-Base Balance
Background:
- Plasma glutamine is a crucial amino acid involved in various metabolic processes.
- Understanding factors influencing glutamine homeostasis is vital for metabolic research.
Purpose of the Study:
- To investigate the effect of chronic metabolic acidosis and alkalosis on plasma glutamine concentrations in humans.
- To determine the specific role of PCO2 and bicarbonate levels in mediating these changes.
Main Methods:
- Healthy male volunteers were subjected to controlled conditions of metabolic acidosis and alkalosis.
- Plasma glutamine concentrations were measured under different acid-base states.
- Acidosis was experimentally induced using elevated CO2 inhalation to isolate PCO2 effects.
Main Results:
- Metabolic acidosis led to a significant decrease in plasma glutamine.
- Metabolic alkalosis resulted in a significant increase in plasma glutamine.
- Elevated PCO2 levels, independent of bicarbonate changes, caused a significant increase in glutamine.
Conclusions:
- Acid-base balance significantly modulates plasma glutamine concentrations.
- Partial pressure of carbon dioxide (PCO2) is identified as a key mediator of glutamine level alterations during acid-base disturbances.
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