Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Methodological variation in antibiotic synergy tests against enterococci.

R W Ryan, I Kwasnik, R C Tilton

    Journal of Clinical Microbiology
    |January 1, 1981
    PubMed
    Summary

    The synergy screen accurately predicted antibiotic synergy in enterococci, unlike the variable microdilution checkerboard method. High-level aminoglycoside resistance screening is recommended for enterococci.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Medical Aspects of the Air Force.

    Canadian Medical Association journal·2010
    Same author

    Some Aspects of Aviation Medicine.

    Canadian Medical Association journal·2010
    Same author

    [Diagnostic efforts for the detection of chlamydia trachomatis infections in Iceland 1982-1994.].

    Laeknabladid·2010
    Same author

    Culture Borrelia burgdorferi.

    Journal of clinical microbiology·2001
    Same author

    Antibiotic resistance: the Bermuda experience.

    Journal of clinical microbiology·1999
    Same author

    Outcomes assessment of a residency program in laboratory medicine.

    Annals of clinical and laboratory science·1998

    Area of Science:

    • Microbiology
    • Infectious Diseases
    • Pharmacology

    Background:

    • Enterococci are significant human pathogens.
    • Antibiotic synergy is crucial for treating enterococcal infections.
    • Accurate methods for detecting antibiotic synergy are needed.

    Purpose of the Study:

    • To evaluate three methods for detecting antibiotic synergy in enterococci.
    • To compare the reliability of synergy screen, microdilution checkerboard, and time-kill curves.
    • To identify an optimal method for screening enterococcal antibiotic synergy.

    Main Methods:

    • Testing 32 human enterococcal isolates for antibiotic synergy.
    • Utilizing penicillin combined with six aminoglycosides.
    • Employing synergy screen, microdilution checkerboard, and time-kill curve assays.

    Main Results:

    • Synergy screen accurately predicted gentamicin-penicillin synergy, confirmed by time-kill curves.
    • Microdilution checkerboard showed significant variation and poor agreement with time-kill curves.
    • A proposed screening criterion using 2,000 µg/mL aminoglycoside demonstrated effectiveness.

    Conclusions:

    • The synergy screen is a reliable method for predicting antibiotic synergy in enterococci.
    • The microdilution checkerboard method is not recommended due to inherent variability.
    • Screening for high-level aminoglycoside resistance is a valuable approach for enterococci.

    Related Experiment Videos