[Types of 3d cerebral ventricle ependyma in sheep and their localization using segment analysis]

Attention was focused to the determination of the topographical locality of different ependyma types and determination of their extent by the method of segment analysis. Brain samples of five sheep of the Slovak Merino breed were studied. Excised brains were transversally cut into segments from the frontal pole at 5mm intervals. The eighth, ninth and tenth slices were cast in paraffin and were cut in simultaneous series at 300 microns intervals, the cutting thickness being 10 microns. The paraffin slices were stained with haematoxylin-eosine, Gömöri's chromium haematoxylin, and by the aldehyde fuchsine method after Gömöri. Nine types of ependymal epithelium of the third cerebral ventricle were observed, according to the kind and arrangement of cells. Remarkable differences were found in the occurrence of the types of cells and in their arrangement in the ependyma at the level of the arch of the side walls and bottom of the ventricle within each segment and within the extent of the studied transversal cuts. From the viewpoint of the regulation of hypophysial functions, a significant difference exists in the structure and organization of ependyma at the locality of recessus supraopticus, recessus infundibularis and recessus mammillaris. The results of our study are in agreement with findings in laboratory animals and enrich our previous findings.