Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phosphoprotein modulation of apatite crystallization.

J D Termine, E D Eanes, K M Conn

    Calcified Tissue International
    |January 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Amorphous Calcium Phosphate-Based Bioactive Polymeric Composites for Mineralized Tissue Regeneration.

    Journal of research of the National Institute of Standards and Technology·2016
    Same author

    Environmental exposures and respiratory morbidity among very low birth weight infants at 1 year of life.

    Archives of disease in childhood·2008
    Same author

    Dental composites based on hybrid and surface-modified amorphous calcium phosphates.

    Biomaterials·2003
    Same author

    The selective estrogen receptor modulator raloxifene regulates osteoclast and osteoblast activity in vitro.

    Bone·2002
    Same author

    Amorphous calcium phosphate.

    Monographs in oral science·2002
    Same author

    Role of OCP in biological processes.

    Monographs in oral science·2002

    Phosphoproteins inhibit apatite crystal growth by prolonging initial lag periods and delaying the conversion of octacalcium phosphate. Calcium and collagen reduce this inhibitory effect, suggesting phosphoproteins regulate dentin mineralization.

    Area of Science:

    • Biomineralization
    • Crystallography
    • Biochemistry

    Background:

    • Dentin mineralization is a complex biomineralization process.
    • Phosphoproteins are key organic components implicated in regulating mineral formation.
    • Understanding phosphoprotein interactions is crucial for comprehending dentin development and pathology.

    Purpose of the Study:

    • To investigate the inhibitory effects of various phosphoprotein preparations on apatite crystal growth.
    • To elucidate the mechanisms by which phosphoproteins modulate mineral formation in vitro.
    • To explore the influence of calcium and collagen on phosphoprotein-mediated inhibition.

    Main Methods:

    • Inhibition assays using pre-existing apatite seeds in metastable solutions.
    • Evaluation of phosphoprotein effects on initial lag phase and mineral conversion stages.

    Related Experiment Videos

  • Assessment of de novo precipitation in synthetic extracellular fluids.
  • Treatment of phosphoproteins with calcium and collagen to determine their impact.

    • Main Results:

    • Phosphoproteins (phosvitin, dentin phosphoproteins) significantly inhibited apatite growth by extending the lag phase and delaying octacalcium phosphate conversion.
    • Pre-treatment with calcium reduced the inhibitory capacity of phosphoproteins on both seeded growth and de novo formation.
    • Collagen diminished inhibition of de novo precipitation but did not affect phosphoprotein-modulated seeded crystal growth.

    Conclusions:

    • Phosphoproteins play a significant regulatory role in dentin mineralization by controlling apatite crystal nucleation and growth.
    • The inhibitory activity of phosphoproteins is modulated by calcium ions and the presence of collagen.
    • These findings provide insights into the molecular mechanisms governing biomineralization in hard tissues.