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[Clofoctol binding by the bacteria (author's transl)].

J Combe, F Simonnet, F Yablonsky

    Journal De Pharmacologie
    |October 1, 1980
    PubMed
    Summary
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    Clofoctol is a fast-acting antibacterial agent effective against Gram-positive bacteria. Its binding mechanism involves hydrophobic interactions, unaffected by ions or pH, but inhibited by sodium lauryl sulfate.

    Area of Science:

    • Microbiology
    • Pharmacology
    • Biochemistry

    Background:

    • Clofoctol exhibits antibacterial activity, primarily against Gram-positive bacteria.
    • Understanding the binding kinetics and characteristics of clofoctol is crucial for its therapeutic application.

    Purpose of the Study:

    • To investigate the binding mechanism of clofoctol to bacterial cells.
    • To determine factors influencing clofoctol binding, including environmental conditions and cell viability.

    Main Methods:

    • Quantitative analysis of clofoctol binding to bacterial cells using radiolabeled (14C) and unlabeled clofoctol.
    • Assessment of binding in the presence of various ions (K+, Mg++, Ca++, Mn++, Fe++), pH variations, urea, and sodium lauryl sulfate.
    • Experiments conducted with both viable and thermally inactivated bacterial cells, including Gram-positive and Gram-negative protoplasts.

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    Main Results:

    • Clofoctol binding is rapid, with approximately 10^6 to 1.5 x 10^7 molecules binding per bacterium.
    • Binding occurs with both viable and inactivated cells, as well as Gram-positive and Gram-negative protoplasts.
    • Binding is reversible and unaffected by a wide range of pH (2-9), common ions, and urea, but is inhibited by sodium lauryl sulfate.

    Conclusions:

    • Clofoctol binding to bacteria is mediated by hydrophobic interactions.
    • The sensitivity of bacteria to clofoctol is directly related to their capacity for binding the agent.
    • The binding characteristics suggest a specific interaction independent of major cellular metabolic processes.