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Updated: Aug 6, 2026

Bacterial Immobilization for Imaging by Atomic Force Microscopy
Published on: August 10, 2011
[Effect of electro-immobilization on Bacillus subtilis]
Abstract:
The culture of Bacillus subtilis 21 was subjected to the action of nonuniform electric field, and the effect of the latter on the bacterial survival and biochemical activity was studied. The action of the field on the cells was shown to depend on the material of a load on which the culture was immobilized. The studied properties of Bac. subtilis 21 did not change when the culture was immobilized on cellulose fiber. About 50--60% of the cells died on silica gel under the action of field; the respiration activity and the rate of hexamethylene diamine destruction did not change. Almost all of the bacterial cells lost their viability upon electroimmobilization on ion-exchange resins. The destructive properties of the culture retained by the field exceed the activity of the control variants.
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