[Activity of acid hydrolases in rat tissues under experimental arthritis]

The enzymatic activity of acid cathepsins, hyaluronidase, beta-D-glucuronidase, alpha-D- and beta-D-glucosidases in blood serum as well as some of these activities in the synovial fluid, liver tissue and spleen were determined in different periods of adjuvant arthritis, in rats. In the first days of arthritis (the acute stage) in rats the activity of hyaluronidase, beta-D-glucuronidase, and acid cathepsins in blood serum, that of hyaluronidase and acid cathepsins in the synovial fluid as well as of acid cathepsins in the spleen tissues were increased. The found inhibition of the beta-D-glucosidase activity in blood serum, synovial fluid and spleen tissue, alpha-D-glucosidase activity in the liver tissue against a background of activation of acid cathepsins, hyaluronidase, beta-D-glucuronidase in blood serum, of acid cathepsins, hyaluronidase, beta-D-glucuronidase in the synovial fluid as well as acid cathepsins in the spleen is associated with the further development of the pathological process and characterizes the adjuvant arthritis chronic stage. The alpha-D-glucosidase activity in blood serum is exception, its inhibition is manifested from the third day of arthritis.