Amino acid sequence of a myoglobin isolated from map turtle, Graptemys geographica

Myoglobin was isolated from the skeletal muscle of map turtle, Graptemys geographica, and purified by Sephadex G-75 and DEAE-cellulose column chromatography. The myoglobin was cleaved by cyanogen bromide treatment, by peptic digestion, and by tryptic digestion of myoglobin whose lysine residues had been modified with citraconic anhydride or 1,2,4-benzenetricarboxylic anhydride. From the amino acid sequences of these fragments, the complete amino acid sequence was deduced.