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Exchanges between plasma and red cells at a variable temperature.

J L Rodeau, A Malan

    Respiration Physiology
    |December 1, 1980
    PubMed
    Summary
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    The difference between plasma and red blood cell pH remains constant when blood temperature changes under anaerobic conditions. This stability is mainly due to CO2 diffusion and ion equilibrium, not just buffer composition.

    Area of Science:

    • Physiology
    • Biochemistry
    • Blood gas analysis

    Background:

    • Understanding blood pH regulation is crucial for physiological homeostasis.
    • Temperature significantly influences blood gas parameters and acid-base balance.

    Purpose of the Study:

    • To investigate the relationship between blood temperature and the pH difference between plasma and red blood cells under anaerobic conditions.
    • To elucidate the underlying mechanisms responsible for maintaining this pH difference.

    Main Methods:

    • Experimental manipulation of blood temperature in closed (anaerobic) systems.
    • Utilizing model studies to analyze diffusive CO2 equilibration and ion (HCO3-, Cl-) movement.
    • Comparing the influence of buffer composition versus diffusive and equilibrium factors.

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    Main Results:

    • A remarkable constancy was observed in the plasma pH minus red cell pH difference across varying blood temperatures.
    • Model simulations indicated that CO2 diffusion and Donnan equilibrium of bicarbonate and chloride ions are the primary drivers of this pH constancy.
    • The role of a strictly defined buffer composition was found to be less significant than these equilibrium processes.

    Conclusions:

    • The observed stability in the red cell-plasma pH gradient under changing temperatures is primarily governed by physical-chemical principles of diffusion and ion exchange.
    • These findings highlight the dynamic regulation of intracellular and extracellular pH in response to temperature shifts, independent of fixed buffer concentrations.