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Evolution in Pseudomonas fluorescens.

A B Champion, E L Barrett, N J Palleroni

    Journal of General Microbiology
    |October 1, 1980
    PubMed
    Summary
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    This study explored Pseudomonas fluorescens relationships using phenotypic, DNA, and azurin protein analyses. Genotypic and phenotypic data largely agreed, offering insights into bacterial evolution and gene transfer.

    Area of Science:

    • Microbiology
    • Bacterial genetics
    • Evolutionary biology

    Background:

    • Pseudomonas fluorescens is a ubiquitous bacterium with diverse strains.
    • Understanding the evolutionary relationships within bacterial species is crucial for microbiology.
    • Genotypic and phenotypic variations can provide insights into evolutionary processes.

    Purpose of the Study:

    • To investigate the phylogenetic relationships among 93 strains of Pseudomonas fluorescens.
    • To compare the congruence between genotypic (DNA hybridization, azurin immunofixation) and phenotypic data.
    • To explore the roles of horizontal and vertical gene transfer in bacterial evolution.

    Main Methods:

    • Numerical taxonomic analysis of 150 phenotypic tests.
    • DNA hybridization studies with 16 reference strains.

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  • Quantitative microcomplement fixation using antibodies against the azurin protein.

    • Main Results:

    • The 93 Pseudomonas fluorescens strains formed distinct clusters based on the analyses.
    • Azurin immunological similarity showed 98% agreement with DNA homology, indicating conserved genetic patterns.
    • 88% of genotypically clustered strains also clustered phenotypically, with discrepancies attributed to variable phenotypic evolution.

    Conclusions:

    • Genotypic and phenotypic data provide a robust framework for understanding Pseudomonas fluorescens relationships.
    • The findings suggest significant roles for both horizontal and vertical gene transfer in the evolution of this bacterial group.
    • Azurin protein evolution appears to closely mirror overall genomic evolution in Pseudomonas fluorescens.