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Urinary tract infection after injection of pseudomonas into bladder
Abstract:
Pseudomonas urinary tract infection was studied in female mice. The bladder was exposed by a lower midline incision and an overnight washed culture of Pseudomonas (0.05 ml.) was injected into the bladder. This resulted in infection of the kidneys in more than 50 per cent of mice at one week. At four hours, the microulceration of the bladder was observed with many Pseudomonas int the base of the ulcer. The presence of acute inflammation on the serosal surface of the bladder, the frequent death of the mice, the presence of cortical foci of polymorphonuclear leukocytes in the kidneys, and bacteremia suggested that bacteremia may play a significant role in the development of the renal infection in this model of urinary tract infection. Bacteria injected through the bladder wall of mice cannot be assumed to produce ascending infection of the kidney.
Insights
This study investigated Pseudomonas urinary tract infections in female mice, finding that bladder infections led to kidney infections in over 50% of mice. Bacteremia appears crucial for developing renal infections in this model.
Area of Science:
- Microbiology
- Urology
- Infectious Diseases
Background:
- Urinary tract infections (UTIs) are common bacterial infections.
- Pseudomonas species are known opportunistic pathogens that can cause UTIs.
- Understanding the pathogenesis of Pseudomonas UTIs is crucial for effective treatment.
Purpose of the Study:
- To investigate the development of Pseudomonas urinary tract infections in a murine model.
- To determine the role of bacteremia in the progression of renal infection.
- To examine the pathological changes in the bladder and kidneys during Pseudomonas infection.
Main Methods:
- Female mice were inoculated with Pseudomonas directly into the bladder via surgical exposure.
- Bladder and kidney tissues were examined for bacterial presence and pathological changes.
- Bacteremia was assessed to evaluate systemic spread of infection.
Main Results:
- Bladder inoculation led to kidney infections in over 50% of mice within one week.
- Microscopic ulcerations and acute inflammation were observed in the bladder.
- Cortical foci of polymorphonuclear leukocytes and bacteremia were present in infected kidneys, suggesting systemic spread.
- Bacterial injection through the bladder wall did not necessarily result in ascending kidney infection.
Conclusions:
- Bacteremia plays a significant role in the development of renal infections in this Pseudomonas UTI model.
- Pseudomonas UTIs can lead to severe pathology in both the bladder and kidneys.
- This model provides insights into the pathogenesis of Pseudomonas-induced UTIs and highlights the importance of considering systemic spread.