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Resistance of Pseudomonas aeruginosa to modern eye-banking technique
Abstract:
Rabbit eyes were inoculated with different concentrations of Pseudomonas aeruginosa. Copious irrigation and topical Neosporin solution decreased surface contamination by 90% as compared to controls. Ps. aeruginosa added to M-K medium and stored at 4 degree C remained viable for 6 days and multiplied rapidly when the medium was incubated at 35 degree C. Adding penicillin and streptomycin (125 units/ml each) suppressed, but did not prevent this growth. Gentamicin (100 micrograms/ml) suppressed growth in all vials by 72 hours at 4 degree C. These studies show that gentamicin is clearly superior to penicillin and streptomycin in preventing Ps. aeruginosa contamination of donor corneas. It is suggested that donor eyes and M-K media be routinely cultured upon arrival at the eye-bank and the time of surgery in order to avoid or immediately treat gentamicin-resistant donor cornea contamination.
Insights
Gentamicin effectively prevents Pseudomonas aeruginosa contamination in donor corneas, outperforming penicillin and streptomycin. Routine culturing of donor eyes and media is recommended to manage potential contamination.
Area of Science:
- Ophthalmology
- Microbiology
- Transplantation Science
Background:
- Pseudomonas aeruginosa is a significant cause of microbial keratitis and donor cornea contamination.
- Effective antimicrobial strategies are crucial for preventing contamination in eye banking.
Purpose of the Study:
- To evaluate the efficacy of gentamicin, penicillin, and streptomycin in preventing Pseudomonas aeruginosa contamination in donor corneas.
- To assess the viability and growth of Pseudomonas aeruginosa in culture media under different temperature conditions.
Main Methods:
- Rabbit eyes were inoculated with Pseudomonas aeruginosa.
- Antimicrobial efficacy of gentamicin, penicillin, and streptomycin was tested.
- Pseudomonas aeruginosa growth was monitored in M-K medium at 4°C and 35°C.
Main Results:
- Topical Neosporin and irrigation reduced surface contamination by 90%.
- Pseudomonas aeruginosa remained viable for 6 days at 4°C and multiplied rapidly at 35°C.
- Gentamicin (100 µg/ml) suppressed growth by 72 hours at 4°C, demonstrating superiority over penicillin and streptomycin.
Conclusions:
- Gentamicin is significantly more effective than penicillin and streptomycin in preventing Pseudomonas aeruginosa contamination of donor corneas.
- Routine culturing of donor eyes and M-K media upon arrival and before surgery is recommended to detect and treat contamination.
- This approach can help avoid or manage gentamicin-resistant donor cornea contamination.