[Isolation of DNA-membrane complexes from Bacillus subtilis]

The DNA-membrane complexes were isolated from the membranes of Bacillus subtilis, disrupted by ultrasonication and subjected to chromatography on Sepharose 4B. The membrane-bound fraction of native 3H-DNA was found only in the freshly isolated material. The phospholipid and protein composition of this fraction was determined. It was assumed that the newly synthesized membrane-associated 3H-DNA from Bacillus subtilis is bound to the lipoprotein complexes enriched with phospholipids.