Related Experiment Videos
Mediator production and tumorigenesis of murine monocyte lines
Abstract:
A murine macrophage-like cell line P388D1 (PD1) and its subline PD2 were studied for mediator production and also for tumorigenic properties. When PD1 cells were cultured in a medium containing 5% fetal calf serum, the culture supernatants exhibited potent interleukin 1 (IL1) activity (also termed lymphocyte-activating factor). IL1 activity produced in unstimulated cultures was predominantly of a low-molecular-weight species (mol wt, 13,000-16,000), but in PD1 cultures stimulated with 10-20 microgram lipopolysaccharide/ml a major IL1 activity was associated with a substance(s) of 65,000-75,000 in molecular weight. The PD1 cell line was nontumorigenic in normal female, syngeneic, inbred DBA/2J mice but produced progressively growing tumors in animals that had been immunosuppressed by 450-rad whole-body X-irradiation and treatment with antithymocyte serum. Inoculation with PD1 failed to produce tumors in athymic nude mice, and the spleens of nude mice that rejected PD1 grafts contained significant levels of a theta-positive cell population(s). PD2, a nonadherent and nonphagocytic subline of PD1, produced very little IL1 but instead produced a potent lymphocyte-inhibiting factor in vitro. The PD2 cell line was highly tumorigenic in syngeneic DBA/2J hosts. These results indicated a direct association between tumorigenicity and the type of mediators produced by these murine macrophage cell lines.
Insights
Murine macrophage cell lines showed that producing interleukin 1 (IL1) was linked to non-tumorigenicity, while producing lymphocyte-inhibiting factors correlated with high tumorigenicity in mice.
Area of Science:
- Immunology
- Cell Biology
- Cancer Research
Background:
- Murine macrophage cell lines P388D1 (PD1) and its subline PD2 were investigated for their mediator production and tumorigenic potential.
- Understanding the relationship between immune mediators and tumor growth is crucial in cancer research.
Purpose of the Study:
- To investigate the association between the type of mediators produced by murine macrophage cell lines and their tumorigenic properties.
- To characterize the interleukin 1 (IL1) activity and lymphocyte-inhibiting factors produced by PD1 and PD2 cell lines.
Main Methods:
- Cell culture of P388D1 (PD1) and PD2 murine macrophage cell lines.
- Analysis of mediator production (IL1, lymphocyte-inhibiting factor) in culture supernatants using molecular weight determination.
- Assessment of tumorigenicity by inoculating cell lines into syngeneic DBA/2J mice, immunosuppressed mice, and athymic nude mice.
Main Results:
- PD1 cells produced potent interleukin 1 (IL1) activity, with molecular weight varying based on stimulation (lipopolysaccharide).
- PD1 cells were nontumorigenic in normal mice but formed tumors in immunosuppressed mice, and were rejected by athymic nude mice.
- PD2 cells produced minimal IL1 but significant lymphocyte-inhibiting factor, and were highly tumorigenic in syngeneic mice.
Conclusions:
- A direct association exists between the type of mediators produced by murine macrophage cell lines and their tumorigenicity.
- Interleukin 1 (IL1) production may be inversely related to tumorigenicity, while lymphocyte-inhibiting factors correlate with tumor growth.