[Determination of glucoamylase activity using an enzyme electrode]

Both, the differing definitions of glucoamylase activity and the different determination of the fragment, glucose, preclude a comparison of literature data. Specific enzymatic determination of the fragment, glucose, with an enzyme electrode is proposed. The contribution describes the manufacture of an enzyme electrode from a commercial pO2-electrode and immobilized GOD. The measurement may be carried out by kinetic determination of utilized oxygen or oxygen peroxide formed. The continuous measurement proposed for serial experiments is made via a calibration curve, whose regression coefficient was determined to be 0.999. Depending on the enzyme activity, the reaction time is between 15 s and 3 min.