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Unique V kappa group associated with two mouse L chain genetic markers
Summary
Genetic differences on mouse chromosome 6 influence kappa light chain variable region (V kappa) expression. A new V kappa group, V kappa (Ser), and specific markers like IB-peptide and IgK-Ef1a are identified, revealing distinct V kappa repertoires.
Area of Science:
- Immunogenetics
- Molecular immunology
- Mouse genetics
Background:
- Congeneic mouse strains C.C58 and C.AKR differ from BALB/c at chromosome 6 loci.
- These loci control kappa light chain variable region (V kappa) polymorphisms and Lyt-2/Lyt-3 alloantigens.
Purpose of the Study:
- To investigate V kappa repertoire differences between mouse strains.
- To characterize novel V kappa groups and genetic markers associated with chromosome 6.
Main Methods:
- Amino acid sequence analysis of myeloma light chains.
- Peptide mapping.
- Isoelectric focusing of myeloma light chains.
Main Results:
- A novel V kappa group, V kappa (Ser), was identified, characterized by an NH2-terminal serine.
- The M75 light chain contained the IB-peptide marker (IgK-Trpa allele) and corresponded to IgK-Ef1a.
- Additional C.C58 and C.AKR myeloma light chains confirmed the V kappa (Ser) group and IgK-Ef1a association.
Conclusions:
- Chromosome 6 genes control the expression of distinct V kappa repertoires.
- The IB-peptide and IgK-Ef1a markers represent differences in V kappa expression, including the V kappa (Ser) group.