[Material on experimental polyembryony in Gallus gallus domestica chickens]

Cutting the blastodisc of nonincubated chick eggs in parallel and perpendicularly to the presumptive axis results in the development of twin embryos in 28% of cases. After tripsin and versen solutions were injected in various concentrations into the blastula cavity, the part of the developed twins was but negligible (5% and 3%, respectively). In all experimental groups embryos with different developmental anomalies were often occurrence, as well as various types of malformation were noted in twins. Histological investigation of embryos having teratomic character demonstrated double anlage of some organs in them. Formation of several neural tubes, chordae and hearts was noted. It is possible that in most cases somatic embryogenesis takes place, incompleted due to intensive processes of regulatory character at early embryogenesis in avian. Results of in vitro experiments for studying regulatory capability of early chicken embryos (mechanical trauma was performed in the embryo for this purpose) demonstrate a rather high degree of integration that should prevent processes of polyembryony to develop in the experiments.