Regulatory properties of the pyruvate dehydrogenase complex of Pseudomonas aeruginosa

The pyruvate dehydrogenase multienzyme complex of Pseudomonas aeruginosa was subjected to a steady-state kinetic analysis using the exponential model for a regulatory enzyme and a sensitive statistical fitting procedure. This showed that all the substrates, pyruvate, CoA and NAD+, exhibit cooperative kinetics towards the native multienzyme complex.