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DNA-histone interactions in nucleosomes.

K E Van Holde, J R Allen, K Tatchell

    Biophysical Journal
    |October 1, 1980
    PubMed
    Summary
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    DNA interacts with the histone core, with approximately 168 base pairs (bp) binding. Different DNA regions exhibit varying binding strengths, influencing nucleosome structure and stability.

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Genetics

    Background:

    • Nucleosomes are the fundamental units of DNA packaging in eukaryotes.
    • Understanding DNA-histone interactions is crucial for gene regulation and chromatin structure.

    Purpose of the Study:

    • To investigate the binding characteristics of DNA to the histone core of the nucleosome.
    • To determine the total length of DNA interacting with the histone core and the nature of these interactions.

    Main Methods:

    • Micrococcal nuclease digestion.
    • Thermal denaturation studies.
    • Theoretical analysis of DNAse I digestion patterns.

    Main Results:

    • Approximately 168 base pairs (bp) of DNA interact with the histone core.

    Related Experiment Videos

  • DNA-histone interactions are categorized into three classes based on binding strength and ionic strength dependence.
  • A central 100 bp region shows strong binding, while outer regions are more weakly associated.
  • Histone core protection patterns are consistent between isolated core particles and those within nuclei.

    • Conclusions:

    • The histone core can bind approximately 168 bp of DNA, with distinct binding affinities for different DNA segments.
    • These findings provide insights into the structural organization and stability of nucleosomes.
    • The DNA-histone core interaction model is conserved across different cellular contexts.