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[Preparation of specific pituitary cells by centrifugal elutriation]
Abstract:
The cells of the male rat pituitary were enzymatically dispersed with trypsin and then separated by centrifugal elutriation. The commercial equipment requires minor modifications, especially the infection system. Using a gradient of flow, enriched gonadotrop cells are obtained. The results compare favourably with those obtained by sedimentation at unit gravity.