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A novel lymphocyte function-associated antigen (LFA-1): cellular distribution, quantitative expression, and

K Kürzinger, T Reynolds, R N Germain

Journal of Immunology (Baltimore, Md. : 1950)

|August 1, 1981

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This study characterizes lymphocyte function-associated antigen 1 (LFA-1), a molecule crucial for T cell functions. LFA-1 is a glycoprotein found on T and B cells, with higher expression on T cells, and its structure and distribution distinguish it from other CTL surface antigens.

Area of Science:

Immunology
Cell Biology

Background:

Monoclonal antibody M7/14 inhibits T cell functions like killing and proliferation.
The target antigen of M7/14 is identified as lymphocyte function-associated antigen 1 (LFA-1).

Purpose of the Study:

To investigate the cell distribution, surface abundance, and structure of LFA-1.
To compare LFA-1 with other T cell surface antigens.

Main Methods:

Flow cytometry and antibody binding assays using M7/14 monoclonal antibody.
Analysis of LFA-1 molecular structure via polypeptide chain composition.

Main Results:

LFA-1 is expressed on T and B lymphoid cells and bone marrow cells, but not macrophages or nonlymphoid tissues.
T cells exhibit higher LFA-1 expression than B cells.

Related Experiment Videos

LFA-1 is a glycoprotein composed of 180,000 and 95,000 Mr polypeptide chains, distinct from other known CTL surface antigens.

Conclusions:

LFA-1 plays a specific role in T cell function, distinct from antigens like Lyt-2,3.
The molecular structure and cell distribution of LFA-1 differentiate it from other T cell surface markers.