Related Experiment Video

Updated: Aug 10, 2026

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
10:32

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag

Published on: January 16, 2012

[Purification and specificity control of labelled antibodies (author's transl)]

H Franz, J Mohr

    Acta Histochemica. Supplementband
    |January 1, 1980
    PubMed

    A review is given about causes of unspecific binding and the possibility of its prevention. Unspecific binding can be caused by: binding of proteins and glycoproteins on cells or tissues by non-covalent links, undesirable antigen-antibody-reactions, non-immune affinity reactions, changes of the charge of antibodies depending on the labelling procedure. A method for fractionation of labelled antibodies by using precipitation with protamine sulphate is described.

    More Related Videos

    Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads06:48

    Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads

    Published on: September 15, 2016

    Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System10:50

    Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

    Published on: May 1, 2019

    Related Concept Videos

    Antibody Structure01:10

    Antibody Structure

    Overview
    Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
    The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
    Antibodies consist of four polypeptide chains: two identical heavy...
    Immunoprecipitation01:20

    Immunoprecipitation

    Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
    Chromatin Immunoprecipitation
    Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...