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Related Experiment Videos

Atypical Escherichia coli in streams.

R C Rychert, G R Stephenson

    Applied and Environmental Microbiology
    |May 1, 1981
    PubMed
    Summary

    Pale yellow colonies on m-FC broth, often Escherichia coli (E. coli), can impact water quality assessments. Recognizing these atypical strains is crucial for accurate stream water analysis and public health protection.

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    Area of Science:

    • Environmental microbiology
    • Water quality assessment
    • Bacteriology

    Background:

    • Standard methods for fecal coliform detection in stream water utilize m-FC broth.
    • Pale yellow colonies frequently appear on m-FC plates, potentially affecting accurate enumeration.

    Purpose of the Study:

    • To investigate the identity and characteristics of pale yellow colonies observed on m-FC broth during stream water analysis.
    • To determine the impact of these atypical colonies on the accuracy of water quality estimations.

    Main Methods:

    • Isolation and identification of pale yellow colonies from m-FC plates using API 20E and serotyping.
    • Culturing atypical strains in nonselective media to assess stress levels.
    • Biochemical testing, including o-nitrophenyl-beta-D-galactopyranoside and gas production in EC medium at 44.5°C.

    Main Results:

    • Over 80% of pale yellow colonies were identified as Escherichia coli (E. coli).
    • These atypical E. coli strains were not environmentally stressed.
    • 50% of atypical E. coli strains were o-nitrophenyl-beta-D-galactopyranoside positive, and 20% produced gas in EC medium.

    Conclusions:

    • Atypical yellow E. coli strains are common in stream waters and can be misidentified.
    • Failure to account for these strains can lead to significant errors in water quality estimations.
    • Further research is needed to refine detection methods for accurate water quality monitoring.

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