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Preaggregation reactions of platelets.

|September 1, 1981

Summary

This summary is machine-generated.

Platelet volume increases before aggregation, contrary to some prior studies. This swelling, due to water uptake, is often masked by experimental conditions like EDTA and centrifugation.

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Area of Science:

Hematology
Cell Biology
Biophysics

Background:

Controversy exists regarding platelet volume changes preceding aggregation.
Resistive-counting and centrifugation methods yield conflicting results on platelet swelling.

Purpose of the Study:

To investigate platelet volume changes during pre-aggregation morphological shifts.
To resolve discrepancies between resistive-counting and centrifugation techniques.

Main Methods:

Platelet sizing using a computerized resistive-particle counter before and after adenosine diphosphate (ADP) incubation.
Experimental manipulation of platelet shape using colchicine, chlorpromazine, and temperature changes.
Assessment of platelet density and serotonin release post-ADP exposure.

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Main Results:

Platelet resistive volume increased by 14% after ADP exposure (p < 0.001) without EDTA.
EDTA significantly inhibited ADP-induced platelet volume increase and temperature-induced shrinking.
Platelet handling, like centrifugation, induced particle swelling, and density decreased after ADP exposure, suggesting water uptake.

Conclusions:

Pre-aggregation reactions are associated with increased platelet particle volume, primarily due to water uptake.
Previous studies using centrifugation or EDTA failed to detect this increase due to inhibitory effects and artifacts.