Human seminal lectin. II. Properties of a lectin like factor in the human seminal plasma

A lectin like haemagglutinin (Human Seminal Lectin - HSL) is present in human seminal plasma. Partial purification and characterization of HSL has been achieved. HSL was shown to be almost completely inactivated at 100 degree C within 15 min. HSL was stable within a pH range of 5 to 10 for one hour, was resistant to trypsin, mercaptoethanol and dithiothreitol and has a molecular weight of about 57 000 daltons and an iso-electric point of about 6.8. HSL activity did not depend on either the incubation temperature or on the availability of free divalent cations such as Ca++,Mg++,or Mn++.