A Pseudomonas aeruginosa mutant non-derepressible for orthophosphate-regulated proteins

Using a rapid screening assay based on the hydrolysis of p-nitrophenylphosphorylcholine, we isolated several mutants of Pseudomonas aeruginosa deficient in the production of phospholipase C. One, designated strain A50N, was also markedly deficient in the synthesis of alkaline phosphatase and several unidentified extracellular proteins. Because strain A50N produces these proteins under conditions of derepression at levels equal to those produced by the parental strain PAO1 grown in medium containing excess phosphate, it appears to have a mutation in a genetic element involved in the derepression of phosphate-repressible proteins.