Nucleoside phosphorylase activity in normal and leukemic cells

Nucleoside phosphorylase (NP) activity was assayed in normal peripheral blood lymphocytes, mature and immature thymocytes fractionated by peanut agglutinability, CLL lymphocytes, and ALL lymphoblasts to determine applicability as an enzymatic cell marker. Circulating lymphocytes had the highest activity, immature thymocytes the lowest, and mature thymocytes were intermediate in NP level. This suggests an increase in NP activity with T-cell maturation. CLL lymphocytes showed very low activity, confirming previous reports. However, the NP activity of the lymphoblasts of 40 patients with B, T, and non-B, non-T ALL was similar to that of normal peripheral blood lymphocytes and could not be used to discriminate between these subgroups of ALL.