Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nucleoside phosphorylase activity in normal and leukemic cells.

I Ben-Bassat, F Brok-Simoni, F Holtzman

    Medical and Pediatric Oncology
    |January 1, 1981
    PubMed
    Summary

    Nucleoside phosphorylase (NP) activity varies across lymphocyte types, increasing with T-cell maturation. However, NP levels in acute lymphoblastic leukemia (ALL) lymphoblasts were similar to normal lymphocytes, limiting its use as a diagnostic marker.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Invasive Aspergillosis in Neutropenic Patients with Hematological Disorders.

    Leukemia & lymphoma·2016
    Same author

    Haemoglobin O in An Arab Family.

    British medical journal·2010
    Same author

    Two cycles of escalated BEACOPP followed by four cycles of ABVD utilizing early-interim PET/CT scan is an effective regimen for advanced high-risk Hodgkin's lymphoma.

    Annals of oncology : official journal of the European Society for Medical Oncology·2009
    Same author

    Ibritumomab tiuxetan (Zevalin) combined with reduced-intensity conditioning and allogeneic stem-cell transplantation (SCT) in patients with chemorefractory non-Hodgkin's lymphoma.

    Bone marrow transplantation·2007
    Same author

    Graft-versus-leukemia in chronic lymphocytic leukemia.

    Bone marrow transplantation·2007
    Same author

    Extramedullary disease and targeted therapies for hematological malignancies--is the association real?

    Annals of oncology : official journal of the European Society for Medical Oncology·2006

    Area of Science:

    • Biochemistry
    • Immunology
    • Cell Biology

    Background:

    • Nucleoside phosphorylase (NP) is an enzyme involved in purine metabolism.
    • Enzymatic cell markers are crucial for differentiating lymphocyte populations and diagnosing hematological malignancies.
    • Previous studies indicated low NP activity in chronic lymphocytic leukemia (CLL) lymphocytes.

    Purpose of the Study:

    • To investigate the applicability of nucleoside phosphorylase (NP) activity as an enzymatic cell marker.
    • To compare NP levels in normal lymphocytes, thymocytes, CLL lymphocytes, and acute lymphoblastic leukemia (ALL) lymphoblasts.

    Main Methods:

    • Assay of nucleoside phosphorylase (NP) activity.
    • Fractionation of thymocytes based on peanut agglutinability to distinguish mature and immature populations.

    Related Experiment Videos

  • Analysis of NP levels in peripheral blood lymphocytes, thymocytes, CLL lymphocytes, and ALL lymphoblasts from 40 patients.

    • Main Results:

    • Circulating lymphocytes exhibited the highest NP activity, while immature thymocytes showed the lowest.
    • Mature thymocytes displayed intermediate NP levels, suggesting a correlation between NP activity and T-cell maturation.
    • CLL lymphocytes confirmed previous findings of very low NP activity.
    • NP activity in lymphoblasts from patients with B, T, and non-B, non-T ALL was comparable to normal peripheral blood lymphocytes.

    Conclusions:

    • Nucleoside phosphorylase (NP) activity increases with T-cell maturation.
    • NP activity in ALL lymphoblasts is not sufficiently distinct from normal lymphocytes to serve as a reliable diagnostic marker for ALL subtypes.