Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Background on animal testing in the drug delivery systems program.

W L Dewey

    NIDA Research Monograph
    |January 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Opioid-like adverse effects of tianeptine in male rats and mice.

    Psychopharmacology·2022
    Same author

    Sensitization of enteric neurons to morphine by HIV-1 Tat protein.

    Neurogastroenterology and motility·2015
    Same author

    Site and mechanism of morphine tolerance in the gastrointestinal tract.

    Neurogastroenterology and motility·2014
    Same author

    Reversal of morphine analgesic tolerance by ethanol in the mouse.

    The Journal of pharmacology and experimental therapeutics·2013
    Same author

    The effect of protein kinase C and G protein-coupled receptor kinase inhibition on tolerance induced by mu-opioid agonists of different efficacy.

    The Journal of pharmacology and experimental therapeutics·2009
    Same author

    A survey of the teaching of pain control at the predoctoral level.

    Anesthesia progress·2009

    Selecting effective long-acting narcotic antagonists requires careful animal model selection. This study outlines a multi-stage screening process, from mouse analgesia tests to monkey self-administration studies, ensuring candidate safety and efficacy.

    Area of Science:

    • Pharmacology
    • Toxicology
    • Animal Models

    Background:

    • Developing long-acting narcotic antagonists is crucial for addiction treatment.
    • Selecting appropriate animal models is essential for evaluating drug candidates.
    • Existing screening methods may not fully capture the efficacy and safety of novel preparations.

    Purpose of the Study:

    • To establish a comprehensive animal model-based strategy for screening and selecting long-acting narcotic antagonist preparations.
    • To validate a multi-step evaluation process for candidate drugs.
    • To ensure the development of safe and effective narcotic antagonist therapies.

    Main Methods:

    • Preliminary screening using the mouse tail-flick test to assess analgesia suppression.
    • Secondary evaluation in monkeys, monitoring urinary excretion rates and blood levels.

    Related Experiment Videos

  • Rigorous testing of promising candidates for interference with morphine self-administration in monkeys.
  • Final toxicological assessments, including injection site and whole-animal studies.

    • Main Results:

    • The mouse tail-flick test provided a rapid initial screen for potential candidates.
    • Monkey studies demonstrated the ability to assess pharmacokinetic profiles and drug interference with opioid use.
    • The multi-stage approach successfully identified and evaluated promising long-acting narcotic antagonist preparations.
    • Toxicological data confirmed the safety profile of the selected candidates.

    Conclusions:

    • A tiered approach using distinct animal models is effective for screening long-acting narcotic antagonists.
    • Combining behavioral, pharmacokinetic, and toxicological assessments ensures comprehensive candidate evaluation.
    • This methodology supports the development of safer and more effective treatments for opioid dependence.