Effect of neutral lipids on the activity of rabbit pulmonary mixed-function oxidase system with cytochrome P-450

With increasing amount of cholesterol or neutral lipids, an increase with subsequent inhibition of the reconstituted rabbit pulmonary mixed-function oxidase (MFO) system activity is observed. Lecithin-cholate micelles formed in the reconstituted system may reverse the inhibitory effect of neutral lipids excess. Neutral lipids do not constitute essential component of the reconstituted MFO system however small quantities of neutral lipids may modify the structure of MFO system components to yield the highest 7-EC O-deethylation activities.