A rapid procedure for the isolation of human IgM myeloma proteins

A two-step rapid procedure for the isolation of human IgM paraproteins from plasma samples is described. It involves gel filtration on Ultrogel AcA 34 followed by anion exchange chromatography on DEAE-Sepharose CL6B. The overall yield of IgM is greater than 70% and the final product is pure as judged by immunoelectrophoresis and SDS-polyacrylamide gel electrophoresis. The procedure is applicable to both euglobulin and cryoglobulin IgM proteins of a wide range of electrophoretic mobilities.