Analysis of perinotochordal materials. I. Studies on proteoglycans synthesis

Perinotochordal proteoglycans have been shown to influence somite chondrogenic differentiation. However, information concerning the composition of the proteoglycan molecules synthesized by the notochord, or the exact type of molecule necessary for the induction of somite chondrogenesis is not known. The results of the present study indicate that the proteoglycan extracted from the 8 day old notochord culture consists of predominantly small proteoglycans, while the large aggregates form less than 30% of the total. The chondroitin sulfate composition also shows a cartilage type of proteoglycan molecules synthesized by the notochord.