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Relapsing experimental allergic encephalomyelitis in the SJL/J mouse.
Laboratory Investigation; a Journal of Technical Methods and Pathology
|September 1, 1981
Summary
A single injection of spinal cord homogenate can induce experimental allergic encephalomyelitis (EAE) in mice. However, two injections significantly increased EAE severity and relapses, highlighting the impact of immunization strategy.
Area of Science:
- Neuroimmunology
- Autoimmune Diseases
- Experimental Models
Background:
- Experimental allergic encephalomyelitis (EAE) is a crucial animal model for studying demyelinating diseases like multiple sclerosis.
- Optimizing EAE induction protocols is essential for reliable disease modeling and therapeutic testing.
Purpose of the Study:
- To establish a dose-response curve for single-injection EAE induction in SJL/J mice.
- To compare the efficacy of single versus double injection protocols for EAE induction and disease severity.
Main Methods:
- SJL/J mice were injected with varying doses of syngeneic spinal cord homogenate and Mycobacterium tuberculosis H37RA in Freund's adjuvant.
- Disease severity, relapse rates, and neurological deficits were assessed.
- A comparison was made between single-injection and two-injection (1 week apart) protocols.
Main Results:
- A single injection of as little as 0.025 mg of spinal cord homogenate induced severe EAE in some mice.
- No single injection dose induced severe EAE in all mice.
- Two injections, one week apart, resulted in severe EAE in all mice, with high rates of relapses and significant neurological deficits.
Conclusions:
- Single-injection EAE induction is feasible but variable in SJL/J mice.
- A two-injection protocol dramatically enhances EAE induction, severity, and relapse frequency.
- Immunization strategy significantly impacts EAE development, offering insights into disease pathogenesis and modeling.