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Microbial colonization of prosthetic devices. II. Scanning electron microscopy of naturally infected intravenous
Abstract:
Forty two nonselected naturally infected intravenous catheters were investigated by Scanning Electron Microscopy (SEM) and usual bacteriological methods. In many catheter samples an amorphous deposited substance could be detected by SEM investigation, mostly associated with the isolation of staphylococci, Acinetobacter calcoaceticus and Pseudomonas aeruginosa. The thickest layers of such a substance were found in catheters infected by coagulase-negative staphylococci. The bacteria seemed to be closely packed and cemented by this matrix. It's possible protective role against the defence mechanisms of the host and chemotherapeutic agents was discussed.
Insights
Scanning Electron Microscopy revealed an amorphous substance on infected intravenous catheters, often with staphylococci and other bacteria. This biofilm may protect microbes from host defenses and treatments.
Area of Science:
- Microbiology
- Biomaterials Science
- Infectious Diseases
Background:
- Intravenous catheters are crucial medical devices but are prone to microbial colonization and biofilm formation.
- Biofilm development on medical implants can lead to persistent infections and treatment challenges.
- Understanding the composition and structure of biofilms is essential for developing effective prevention and treatment strategies.
Purpose of the Study:
- To investigate the ultrastructure of microbial colonization on naturally infected intravenous catheters.
- To identify the types of bacteria associated with amorphous deposits on catheter surfaces.
- To explore the potential role of these deposits in microbial protection.
Main Methods:
- Analysis of 42 nonselected, naturally infected intravenous catheters.
- Application of Scanning Electron Microscopy (SEM) for ultrastructural investigation.
- Standard bacteriological methods for microbial identification.
Main Results:
- SEM revealed an amorphous deposited substance on many catheter samples.
- This substance was frequently associated with staphylococci, Acinetobacter calcoaceticus, and Pseudomonas aeruginosa.
- Thickest layers of the amorphous substance were observed with coagulase-negative staphylococci infections.
- Bacteria appeared densely packed and cemented within this matrix.
Conclusions:
- An amorphous matrix is a significant component of biofilm on infected intravenous catheters.
- This matrix, particularly with coagulase-negative staphylococci, may shield bacteria.
- The findings suggest a potential protective role for this matrix against host defenses and antimicrobial agents.