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Esterase activity of hemoglobins: a conformational probe for difference between HbA and HbS
Progress in Clinical and Biological Research
|January 1, 1981
Abstract:
(1) We have observed esterase of Hb A and Hb S. The reaction is consistent with general imidazole catalysis. (2) The rate f p-NPA hydrolysis by Hb A and Hb S is significantly different, probably due to subtle conformational alterations involving beta His 2 and/or its micro-environment in the mutant. (3) During this reaction, irreversible modification of Hb occurs, involving lysines (predominantly Lys beta 82).