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Human pituitary hormones extraction.

A Salas-Valdés, S Nieto-Castillo, V M Sanchez-Hidalgo

    Archivos De Investigacion Medica
    |January 1, 1981
    PubMed
    Summary
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    This study validates a human pituitary hormone extraction method, finding it efficient for isolating growth hormone (GH) and glycoproteins like thyroid-stimulating hormone (TSH). The technique minimizes GH contamination in glycoprotein fractions, ensuring high-quality hormone yields.

    Area of Science:

    • Endocrinology
    • Biochemistry
    • Protein Chemistry

    Background:

    • Accurate extraction of pituitary hormones is crucial for research and therapeutic applications.
    • Previous methods may have limitations in purity and yield of specific hormones.
    • Standardization of extraction protocols ensures reproducibility and reliability.

    Purpose of the Study:

    • To evaluate the efficiency and reproducibility of a specific human pituitary hormone extraction method.
    • To quantify the yields of key pituitary hormones, including growth hormone (GH), follicle-stimulating hormone (FSH), luteinizing hormone (LH), and thyroid-stimulating hormone (TSH).
    • To assess the purity of extracted hormone fractions, particularly regarding GH contamination in glycoprotein-rich fractions.

    Main Methods:

    • Human pituitaries were acetone-fixed and delipidized.

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  • Hormone extraction involved sequential acid (0.3 M KCl, pH 5.5) and alkaline (0.1 N NaOH, pH 10.5) treatments with thioethanol.
  • Fractions were analyzed for protein concentration, characterized by disc-gel electrophoresis, and hormonal activities were quantified using radioimmunoassay.

    • Main Results:

    • Extraction yields and protein concentrations were comparable to established methods.
    • Disc-gel electrophoresis showed reproducible patterns for GH and variations in glycoprotein fractions.
    • Radioimmunoassays indicated low GH contamination in glycoprotein fractions, with significant TSH activity present.
    • Calculated hormonal content per pituitary: GH (3 mg), FSH (761 µg), LH (782 µg), TSH (2.939 mg).

    Conclusions:

    • The described human pituitary hormone extraction technique is effective and reproducible.
    • The method successfully minimizes growth hormone contamination in glycoprotein fractions.
    • The extraction yields for TSH were significant, highlighting the method's utility for isolating specific pituitary hormones.