Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Studies of diffusion in calvaria.

W F Neuman, M W Neuman

    Calcified Tissue International
    |January 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Bone matrix allows small molecule diffusion similarly to cartilage. While vitamin D affects water transport, mineral exchange, not matrix permeability, explains calcium and phosphate flux changes.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Unifying concepts of parathyroid hormone action.

    UR [reports]. U.S. Atomic Energy Commission·2014
    Same author

    The uptake of radioactive phosphorus by the calcified tissue of normal and choline-deficient rats.

    The Journal of biological chemistry·2010
    Same author

    The distribution and excretion of uranium following intravenous injection.

    Federation proceedings·2010
    Same author

    Phosphate exchange as the mechanism for adsorption of the radioactive isotope by the calcified tissues.

    The Journal of biological chemistry·2010
    Same author

    Calcium in osteoblast-enriched bone cells.

    Bone·1992
    Same author

    The calcium-buffering phase of bone mineral: some clues to its form and formation.

    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·1987

    Area of Science:

    • Biophysics
    • Bone Biology
    • Physiology

    Background:

    • Understanding molecular transport across bone is crucial for drug delivery and bone metabolism.
    • Isolated calvaria offer a model to study diffusion independent of systemic factors.

    Purpose of the Study:

    • To quantify diffusion rates of small ions and molecules through isolated calvaria.
    • To investigate the impact of specific biochemical interventions on bone matrix permeability.

    Main Methods:

    • Diffusion experiments using isolated calvaria.
    • Measurement of tracer fluxes for various molecules (H2O, 3-O-methylglucose, lactate, sulfate, methylamine, phosphate).
    • Administration of diphosphonate (EHDP) and vitamin D analogs (1,25(OH)2D3) to assess their effects.

    Related Experiment Videos

    Main Results:

    • Small ions and neutral molecules diffused at approximately 2/3 the rate expected compared to cartilage.
    • EHDP did not affect H2O or sulfate diffusion but reduced phosphate flux.
    • Vitamin D deficiency doubled water diffusion; 1,25(OH)2D3 normalized calcium influx without altering water diffusion.

    Conclusions:

    • Bone matrix exhibits permeability to small molecules comparable to cartilage.
    • Observed changes in calcium and phosphate influx are attributable to bone mineral exchange properties, not alterations in matrix permeability.