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Related Experiment Videos

Bacterial mesosomes: method dependent artifacts.

H R Ebersold, J L Cordier, P Lüthy

    Archives of Microbiology
    |September 1, 1981
    PubMed
    Summary
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    Bacterial mesosomes, once thought to be real cell structures, are actually artifacts. Advanced cryofixation techniques reveal these membrane structures do not exist in Bacillus cereus.

    Area of Science:

    • Microbiology
    • Cell Biology
    • Microscopy Techniques

    Background:

    • Mesosomes were historically considered integral components of Gram-positive bacteria.
    • Their role in cellular processes like septum formation was widely accepted.

    Purpose of the Study:

    • To investigate the true occurrence of mesosomes during Bacillus cereus cell division.
    • To determine if mesosomes are genuine cellular structures or preparation artifacts.

    Main Methods:

    • Investigated mesosome formation during vegetative and sporulating cell division in Bacillus cereus.
    • Compared conventional fixation methods with rapid cryofixation followed by freeze-substitution for electron microscopy.

    Main Results:

    • Conventional fixation methods led to the formation of artifactual, pocket-like membrane invaginations, identified as mesosomes.

    Related Experiment Videos

  • Cryofixation and freeze-substitution demonstrated the absence of mesosomes in ultrathin sections.
  • Rapid freezing prevented the formation of these membraneous artifacts.

    • Conclusions:

    • Mesosomes are not real constituents of Bacillus cereus cells.
    • They are artifacts generated during sample preparation for electron microscopy, particularly with conventional fixation techniques.