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Related Experiment Videos

Complement changes during granulocyte adhesion.

R G Strauss, R E Spitzer, A E Stitzel

    Transfusion
    |November 1, 1981
    PubMed
    Summary
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    The granulocyte adherence assay shows neutrophils attach to nylon fibers without complement activation. However, neutrophil attachment likely initiates complement activation, generating chemotactic activity.

    Area of Science:

    • Biomedical Engineering
    • Immunology
    • Hematology

    Background:

    • Filtration leukapheresis is a medical procedure that removes white blood cells from blood.
    • The granulocyte adherence assay is a laboratory method to study how granulocytes (a type of white blood cell) stick to surfaces.
    • The role of the complement system, a part of the immune system, in this process is not fully understood.

    Purpose of the Study:

    • To investigate the granulocyte adherence assay as an in vitro model for filtration leukapheresis.
    • To determine the effects of passing blood through nylon fibers on the complement system.
    • To understand the relationship between neutrophil adherence and complement activation.

    Main Methods:

    • Anticoagulated blood was passed through columns packed with nylon fibers.

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  • The consumption of complement components (C3, C5, C7, C9) in the blood was measured.
  • The presence of the B antigenic determinant of C3 was analyzed.
  • Chemotactic activity generated in the blood was assessed.

    • Main Results:

    • Neutrophil attachment to nylon fibers occurred independently of complement activation.
    • No significant consumption of complement components or cleavage of C3 was observed during blood flow or incubation.
    • Chemotactic activity was generated, suggesting complement activation is initiated by neutrophil adherence.

    Conclusions:

    • The granulocyte adherence assay can model aspects of filtration leukapheresis.
    • Complement activation is not required for initial neutrophil adherence to nylon fibers.
    • Neutrophil adherence to nylon fibers likely triggers complement activation, leading to chemotactic activity.