Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Specific enhancement of mouse CFU-E by mouse transferrin.

I N Rich, G Sawatzki, B Kubanek

    British Journal of Haematology
    |December 1, 1981
    PubMed
    Summary

    Mouse transferrin supports erythropoietic precursor growth better than human transferrin in mouse bone marrow cultures. Optimal concentrations were identified for both, exceeding theoretical needs.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Blood transfusion: influence of transfusion therapy on outcome.

    Current opinion in anaesthesiology·2006
    Same author

    Higher urinary excretion of essential amino acids in preterm infants fed protein hydrolysates.

    Acta paediatrica (Oslo, Norway : 1992)·2005
    Same author

    Erythropoiesis and performance after two weeks of living high and training low in well trained triathletes.

    International journal of sports medicine·2002
    Same author

    Mass spectrometric investigations of human milk oligosaccharides.

    Advances in experimental medicine and biology·2002
    Same author

    Detection of four human milk groups with respect to Lewis-blood-group-dependent oligosaccharides by serologic and chromatographic analysis.

    Advances in experimental medicine and biology·2002
    Same author

    Efficient retrovirus-mediated transduction of primitive human peripheral blood progenitor cells in stroma-free suspension culture.

    Gene therapy·2001

    Area of Science:

    • Hematology
    • Cell Biology
    • Biochemistry

    Background:

    • Transferrin is crucial for iron transport and cellular growth.
    • Erythropoiesis involves the development of red blood cells from precursors.
    • Understanding transferrin's role in erythropoiesis is vital for hematological research.

    Purpose of the Study:

    • To investigate the differential effects of human and mouse transferrins on erythropoietic precursors.
    • To determine optimal concentrations of transferrins for supporting erythropoiesis in vitro.
    • To assess transferrin's impact on early (BFU-E) and late (CFU-E) erythropoietic progenitors.

    Main Methods:

    • Purification of human and mouse transferrins using chromatography.
    • In vitro culture of mouse bone marrow cells.
    • Assessment of colony formation for erythropoietic precursors (BFU-E, CFU-E) and granulocyte/macrophage progenitors.

    Main Results:

    • Mouse transferrin demonstrated higher specificity and colony formation for CFU-E compared to human transferrin.
    • Optimal concentrations for both transferrins were found to be 1.3x10(-13)M and 1.3x10(-10)M.
    • No dose-dependent relationship was observed for erythropoietic burst or granulocyte/macrophage colony formation.

    Conclusions:

    • Mouse transferrin is more effective than human transferrin in supporting CFU-E growth in mouse bone marrow cultures.
    • Specific, supra-theoretical concentrations of transferrin are optimal for erythropoietic precursor proliferation.
    • Transferrin's influence on erythropoiesis is specific to certain precursor stages and cell types.

    Related Experiment Videos