Related Concept Videos

Intrathecal Delivery of a Test Compound to the Rat Central Nervous System05:04

Intrathecal Delivery of a Test Compound to the Rat Central Nervous System

Source: Chen, Y., et al. Intrathecal Delivery of Antisense Oligonucleotides in the Rat Central Nervous System. J. Vis. Exp. (2019)This video demonstrates the delivery of a test compound into the cerebrospinal fluid (CSF) through a catheter implanted in the lumbar spinal column of a rat model. The compound circulates along the CSF to the brain and spinal cord, enters neurons, and offers therapeutic...
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Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY10:28

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

Optical clearing techniques are revolutionizing the way tissues are visualized. In this report we describe modifications of the original Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging-compatible Tissue-hYdrogel (CLARITY) protocol that yields more consistent and less expensive...
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Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System14:55

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System

We describe a method to target drugs to the central nervous system by either implanting a catheter or performing a bolus injection into the right lateral ventricle in mice. We focus specifically on the delivery of antisense oligonucleotides. This technique is readily adaptable to other drugs and to...
61.7K
Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System12:06

Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System

Optic Nerve transection is a widely used model of adult CNS injury. Ninety percent of retinal ganglion cells (RGCs) whose axons are completely transected (axotomy) die within 14 days after axotomy. This model is easily amenable to experimental manipulations and highly...
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Isolation of Mononuclear Cells from the Central Nervous System of Rats with EAE20:51

Isolation of Mononuclear Cells from the Central Nervous System of Rats with EAE

In this video we demonstrate how to isolate mononuclear cells from the central nervous system of rats with experimental autoimmune encephalomyelitis.
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Generation of a Murine Model with a Central Nervous System Infection02:21

Generation of a Murine Model with a Central Nervous System Infection

This video demonstrates the generation of a mouse model with a CNS infection. A suckling pup is injected with the virus both intracranially and intraperitoneally. The virus spreads, infecting the CNS causing neuronal cell death and inflammation. The pup is assessed for symptoms of infection as the disease...
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