[Single step isolation of a hemoglobin binding protein present in chicken plasma]

A one step procedure for the purification of a Chicken hemoglobin binding protein is reported here for the first time. The method includes a single affinity chromatography step on Sepharose coupled to Chicken hemoglobin. The protein obtained is pure, its ability of binding hemoglobin is maintained and the recovery is close to 100%. Moreover, the same affinity system may be used again.