[Casein hydrolysis by immobilized protosubtilin]

A study was made of the kinetics of sodium caseinate hydrolysis by protosubtilin G10X (Bac. subtilis producer) immobilized on silica gel with the aid of glutar dialdehyde. Optimal conditions for making the process were selected (pH 8.5, temperature 60 degree C, substrate concentration 0.3%). The apparent kinetic parameters of the reaction were calculated. An empiric equation that can be used for practical calculation was derived from the Michaelis-Menten equation with the use of the values obtained (Km = 2.48 +/- 0.10 micrometer/ml, Vmax = 25.6 +/- 1.9 micrometer/min/mg conventional protein).