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Updated: Aug 15, 2026

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
Published on: March 16, 2011
Mutagenesis during transformation of Bacillus subtilis. II. An increase in chemically-induced mutations during
Abstract:
During the development of competency in Bacillus subtilis there was an increased sensitivity to methyl methanesulfonate (MMS) treatments. The frequency of reverse mutation also increased among the MMS-revertible markers by a factor of 100 as compared to vegetative cultures. The frequency of 2-aminopurine(AP)-induced mutagenesis was the same in competent and noncompetent cultures. Studies with DNA-polymerase-deficient mutants showed a direct involvement of DNA polymerase I in promoting MMS and transformation-induced mutagenesis in competent cells.
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