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Related Experiment Videos

Insulin-induced proteins in the toad urinary bladder.

M H Cobb, I A Skipski, I M Reich

    The Biochemical Journal
    |October 15, 1981
    PubMed
    Summary

    Insulin rapidly enhances sodium transport in toad bladders, an effect sustained by new protein synthesis. Specific protein induction by insulin may explain this prolonged increase in sodium transport.

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    Area of Science:

    • Physiology
    • Molecular Biology
    • Endocrinology

    Background:

    • Insulin is known to stimulate active sodium transport in the toad urinary bladder.
    • This effect is rapid and long-lasting, suggesting a role for protein synthesis in its maintenance.

    Purpose of the Study:

    • To investigate the role of insulin-induced protein synthesis in the sustained elevation of sodium transport in the toad urinary bladder.
    • To identify specific proteins whose synthesis is modulated by insulin.

    Main Methods:

    • Utilizing radioactive amino acid incorporation to measure protein synthesis in toad bladder mucosal cells.
    • Employing a dual-label technique to differentiate insulin-affected proteins.
    • Assessing the impact of transcription and translation inhibitors on insulin's effect on sodium transport.

    Main Results:

    • Insulin treatment led to a sustained increase in sodium transport, persisting for over 20 hours.
    • Inhibition of transcription or translation abolished the sustained effect of insulin on sodium transport.
    • Insulin specifically increased the incorporation of amino acids into certain soluble and plasma membrane proteins of granular mucosal cells, without affecting overall protein synthesis or amino acid uptake.

    Conclusions:

    • The sustained stimulation of sodium transport by insulin in the toad urinary bladder is dependent on the synthesis of specific proteins.
    • These newly synthesized proteins, particularly those in the soluble and plasma membrane fractions, are likely crucial for maintaining the elevated sodium transport.
    • Insulin's action involves the targeted induction of protein synthesis, contributing to long-term physiological adaptations.

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